BLOCK-iT™ RNAi Designer
The easiest way to design effective RNAi molecules for great results
 
See also:
Synthetics for in vivo RNAi
 
 
 Target Design Options:  Stealth RNAi™ siRNA miR RNAi shRNA siRNA to Stealth RNAi™ siRNA siRNA to shRNA
 
Step 1: Enter an accession number or provide a nucleotide sequence
 
Accession number:
OR
Nucleotide sequence: Enter only A, C, G, T, and U. See the online Help for additional information
Step 2: If you entered an accession number in Step 1, select regions for target design
 
 Open reading frame (ORF)
5' UTR
3' UTR
Step 3: Choose database for Blast
  NOTE: BLAST is used to compare input sequence with sequences in the database to find unique regions against which to design RNAi targets. The databases contain representative gene sequences for that species. Blast databases were updated on March 23, 2013 and the design output reflects the most up-to-date designs.
Step 4: Choose minimum and maximum G/C percentage
 
Minimum G/C percentage: Maximum G/C percentage:  
Step 5: Click "RNAi Design" to design your miR RNAi molecules (for cloning into Invitrogen's pcDNA™6.2-GW/miR and pcDNA™6.2-GW/EmGFP-miR vectors)
 
       
 
How to Order: You must have an account with Invitrogen to order custom oligos. After your oligos have been designed and added to the order form, you must enter a valid customer number that is keyed to your account. If you do not already have an account, contact your local representative to set up an account.