Step 1: Enter an accession number or provide a nucleotide sequence
Accession number:
OR
Nucleotide sequence: Enter only A, C, G, T, and U. See the online Help for additional information
Step 2: If you entered an accession number in Step 1, select regions for target design
Step 3: Choose database for Blast
Human - Homo sapiens
Mouse - Mus musculus
Rat - Rattus norvegicus
Cattle - Bos taurus
Pig - Sus scrofa
Dog - Canis familiaris
Frog - Xenopus laevis
Chicken - Gallus gallus
Fruitfly - Drosophila melanogaster
Worm - Caenorhabditis elegans
Zebrafish - Danio rerio
Mosquito - Anopheles gambiae
No blast
NOTE: BLAST is used to compare input sequence with sequences in the database to find unique regions against which to design RNAi targets. The databases contain
representative gene sequences for that species. Blast databases were updated on March 23, 2013 and the design output reflects the most up-to-date designs.
Step 4: Choose minimum and maximum G/C percentage
Minimum G/C percentage:
20%
25%
30%
35%
40%
45%
50%
55%
60%
65%
70%
75%
80%
85%
90%
Maximum G/C percentage:
20%
25%
30%
35%
40%
45%
50%
55%
60%
65%
70%
75%
80%
85%
90%
Step 5: Click "RNAi Design" to design your miR RNAi molecules (for cloning into Invitrogen's pcDNA™6.2-GW/miR and pcDNA™6.2-GW/EmGFP-miR vectors)
Guarantee: The BLOCK-iT™ RNAi Designer uses an optimized, proprietary algorithm to design miRNA sequences with 100% homology to their target, so that upon processing their activity will result in cleavage of that target. The BLOCK-iT™ RNAi Designer is such an effective tool for the design of miRNAs that if you order oligos corresponding to two miRNAs designed by the BLOCK-iT™ RNAi Designer, we guarantee that at least one will give greater than 70% knockdown of target RNA, given that transfection efficiency in your experiment is at least 80%.